sgRNA
Science & Engineering abbreviation · Genetics
| Stands for | Single guide RNA |
|---|---|
| Categories | Science & Engineering |
sgRNA stands for Single guide RNA, a genetics abbreviation.
Engineered RNA that directs the Cas9 enzyme to a chosen DNA target in CRISPR gene editing. Its first 20 nucleotides match the target site, and the rest forms a scaffold that binds Cas9; designing an sgRNA is often the first step of an editing experiment.
Example: Three sgRNAs targeting exon 2 were tested, and the most efficient one was used for knockout.
Usage notes
Also called guide RNA (gRNA). Natural CRISPR systems use two separate RNAs, crRNA and tracrRNA; Martin Jinek, Jennifer Doudna, Emmanuelle Charpentier and colleagues fused them into a single chimeric guide in 2012. Online design tools score sgRNAs for predicted efficiency and off-target risk.
Related abbreviations
More genetics abbreviations
- PRS – Polygenic risk score
- QTL – Quantitative trait locus
- RFLP – Restriction fragment length polymorphism
- RNA-seq – RNA sequencing
- RNAi – RNA interference
- scRNA-seq – Single-cell RNA sequencing
- shRNA – Short hairpin RNA
- siRNA – Small interfering RNA
- SNP – Single-nucleotide polymorphism
- snRNA – Small nuclear RNA
- SNV – Single-nucleotide variant
- ssDNA – Single-stranded DNA